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Tandem liquid chromatography methods make use of a next pump and smart column switching To optimize utilization from the detector by minimizing downtime connected with column reconditioning.The wavelength of light absorbed relies on the structure on the compound. Light-weight from the ultraviolet to obvious variety is used for absorbance detection

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-hydroxybenzoic acid elutes far more slowly but surely. Though we can solve thoroughly these two solutes applying cellular period that's 16% v/v acetonitrile, we are not able to solve them Should the mobile phase is ten% tetrahydrofuran.Exactly where a solute divides itself amongst two unique solvents since it is a lot more soluble in one than the